Skip to main content
padlock icon - secure page this page is secure

Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata

Buy Article:

$69.00 + tax (Refund Policy)

Summary

Although photodynamic therapy (PDT) has shown great promise for the inactivation of Candida species, its effectiveness against azole-resistant pathogens remains poorly documented. This in vitro study describes the association of Photogem® (Photogem, Moscow, Russia) with LED (light emitting diode) light for the photoinactivation of fluconazole-resistant (FR) and American Type Culture Collection (ATCC) strains of Candida albicans and Candida glabrata. Suspensions of each Candida strain were treated with five Photogem® concentrations and exposed to four LED light fluences (14, 24, 34 or 50 min of illumination). After incubation (48 h at 37 °C), colonies were counted (CFU ml−1). Single-species biofilms were generated on cellulose membrane filters, treated with 25.0 mg l−1 of Photogem® and illuminated at 37.5 J cm−2. The biofilms were then disrupted and the viable yeast cells present were determined. Planktonic suspensions of FR strains were effectively killed after PDT. It was observed that the fungicidal effect of PDT was strain-dependent. Significant decreases in biofilm viability were observed for three strains of C. albicans and for two strains of C. glabrata. The results of this investigation demonstrated that although PDT was effective against Candida species, fluconazole-resistant strains showed reduced sensitivity to PDT. Moreover, single-species biofilms were less susceptible to PDT than their planktonic counterparts.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Candida albicans; Candida glabrata; Photodynamic therapy; fluconazole-resistance; light emitting diode

Document Type: Research Article

Affiliations: 1: Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University – UNESP, Araraquara, SP, Brazil 2: Instituto de Física de São Carlos, University of São Paulo, São Carlos, SP, Brazil

Publication date: March 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more