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Differentiation of Malassezia furfur and Malassezia sympodialis by glycine utilization

: Unterscheidung von Malassezia furfur und Malassezia sympodialis mittels Glycin-Verwertung

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The genus Malassezia has been revised to include six lipophilic species and one nonlipophilic species. These Malassezia species have been investigated to differentiate their morphological and physiological characteristics. However, assimilation of amino acids as a nitrogen source by these species was not well elucidated. In the present study, isolates of Malassezia species were examined with a glycine medium (containing 7–266 mmol glycine, 7.4 mmol KH2PO4, 4.1 mmol MgSO47H2O, 29.6 mmol thiamine, 0.5% Tween-80 and 2% agar) and a modified Dixon glycine medium (0.6% peptone, 3.6% malt extract, 2% ox-bile , 1% Tween-40, 0.2% glycerol, 0.2% oleic acid, 7 mmol glycine and 2% agar). All M. furfur isolates developed on the glycine medium, assimilating glycine at concentrations of at least 7 mmol l−1. However, the other six Malassezia species were unable to grow on the glycine medium. Also, many colonies of M. furfur grew rapidly, within 2–3 days on the modified Dixon glycine medium, although the other six species showed slow and poor development. From these results, it was suggested that M. furfur might be able to utilize glycine as a single nitrogen source, which the other Malassezia species could not. Therefore, glycine medium was recommended for the differentiation of M. furfur from other species of Malassezia.


In der vorliegenden Studie wurde das Wachstum von Malassezia-Stämmen auf Glycin-Medium (enthaltend 7–266 mmol Glycin als alleinige Stickstoffquelle) und aufmodifiziertem Dixon-Medium (enthaltend 7 mmol Glycin) geprüft. Alle Malassezia furfur-Stämme wuchsen auf Glycin-Medium und assimilierten Glycin in mindestens 7 mmolarer Konzentration. Die anderen Malassezia-Arten wuchsen nicht auf Glycin-Medium. Malassezia furfur wuchs ebenfalls rasch auf modifiziertem Dixon-Medium, wogegen die anderen Malassezia-Arten hier nur langsam und schwach wuchsen. Daraus wird gefolgert, dass M. furfur im Gegensatz zu allen anderen Malassezia-Arten Glycin als alleinige Stickstoffquelle nutzen kann. Es wird daher Glycin-Medium als Selektivmedium zur Abtrennung von M. furfur von den anderen Malassezia-Arten vorgeschlagen.
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Keywords: Differenzierung; Glycin-Assimilation; Malassezia furfur; Malassezia sympodialis; differentiation; glycine assimilation

Document Type: Research Article

Affiliations: 1: Department of Dermatology, Teikyo University School of Medicine, Tokyo, 2: Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa, Japan.

Publication date: June 1, 2002

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