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In vitro studies on the activity of amphotericin B and lipid-based amphotericin B formulations against Aspergillus conidia and hyphae

: In vitro-Empfindlichkeit von Aspergillus-Konidien und -Hyphen gegenüber Amphotericin B und Amphotericin B-Lipidpräparationen

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The minimum inhibitory concentrations (MICs) of amphotericin B and lipid-based amphotericin B formulations against isolates of Aspergillus spp. were tested using a broth microdilution method. Twelve isolates of Aspergillus fumigatus, eight of Aspergillus flavus, six of Aspergillus niger and seven of Aspergillus terreus were examined. In addition, an assay for hyphae of Aspergillus spp. was performed since the invasive form is manifested by the appearance of hyphal structures. MICs of hyphae against lipid-based amphotericin B formulations were within three dilutions higher than those against conidia for almost all isolates of Aspergillus spp. (P < 0.01). In contrast, the differences in the in vitro efficacies of amphotericin B were the lowest for Aspergillus spp. This study demonstrates the importance of the type of inoculum used to test antifungal susceptibilities of Aspergillus spp. The significance of these results for in vivo outcome needs to be determined.


Die minimalen Hemmkonzentrationen (MHK) von Amphotericin B und Amphotericin B-Lipidpräparationen gegenüber Aspergillus-Isolaten wurden mittels Mikrodilutionsmethode getestet. Zwölf Isolate von Aspergillus fumigatus, acht von Aspergillus flavus, sechs von Aspergillus niger and sieben von Aspergillus terreus wurden untersucht. Zuzüglich wurde ein Empfindlichkeitstest mit Hyphen durchgeführt, da diese die eigentliche invasive Form der Aspergillose darstellen. Die MHKs der Lipidpräparationen für Hyphen waren im Vergleich zu Konidien immer signifikant erhöht (P  < 0.01). Für Amphotericin B wurden geringere Unterschiede für alle Aspergillus-Isolate festgestellt. Unsere Untersuchungen zeigen signifikante Unterschiede der MHKs in Abhängigkeit von Hyphen oder Konidien als Inokula auf. Die klinische Signifikanz der Ergebnisse muß evaluiert werden.
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Keywords: Amphotericin B; Aspergillus; Empfindlichkeitsprüfung; amphotericin B; colloidal dispersion; kolloidale Dispersion; liposomal formulation; liposomale Formulierung; susceptibility assay

Document Type: Research Article

Affiliations: 1: Department of Hygiene and Social Medicine, University of Innsbruck, 2: Institute of Biostastistics and Documentation, University of Innsbruck, and 3: Division of Haematology, Department of Internal Medicine, Innsbruck University Hospital, Austria.

Publication date: June 1, 2002

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