@article {Harada:2015:0950-382X:1184,
title = "Stereochemical conversion of C3vinyl group to 1hydroxyethyl group in bacteriochlorophyll c by the hydratases BchF and BchV: adaptation of green sulfur bacteria to limitedlight environments",
journal = "Molecular Microbiology",
parent_itemid = "infobike://bsc/mole",
publishercode ="bp",
year = "2015",
volume = "98",
number = "6",
publication date ="2015-12-01T00:00:00",
pages = "1184-1198",
itemtype = "ARTICLE",
issn = "0950-382X",
eissn = "1365-2958",
url = "https://www.ingentaconnect.com/content/bsc/mole/2015/00000098/00000006/art00014",
doi = "doi:10.1111/mmi.13208",
author = "Harada, Jiro and Teramura, Misato and Mizoguchi, Tadashi and Tsukatani, Yusuke and Yamamoto, Ken and Tamiaki, Hitoshi",
abstract = "Photosynthetic green sulfur bacteria inhabit anaerobic environments with very lowlight conditions. To adapt to such environments, these bacteria have evolved efficient lightharvesting antenna complexes called as chlorosomes, which comprise selfaggregated bacteriochlorophyll
c in the model green sulfur, bacterium C
hlorobaculum tepidum. The pigment possess a hydroxy group at the C31 position that produces a chiral center with R or Sstereochemistry and the C31hydroxy group serves as
a connecting moiety for the selfaggregation. C
hlorobaculum tepidum carries the two possible homologous genes for C3vinyl hydratase, bch
F and bch
V. In the present study, we constructed deletion mutants of each of these genes. Pigment
analyses of the bch
Finactivated mutant, which still has BchV as a sole hydratase, showed higher ratios of Sepimeric bacteriochlorophyll c than the wildtype strain. The heightened prevalence of Sstereoisomers in the mutant was
more remarkable at lower light intensities and caused a red shift of the chlorosomal Qy absorption band leading to advantages for lightenergy transfer. In contrast, the bch
Vmutant possessing only BchF showed a significant decrease of the Sepimers
and accumulations of C3vinyl BChl c species. As trans criptional level of bch
V was upregulated at lower light intensity, the C
hlorobaculum tepidum adapted to lowlight environments by control of the bch
V transcription.",
}