Skip to main content
padlock icon - secure page this page is secure

Free Content Cis-acting signals controlling translational initiation in the thermophilic archaeon Sulfolobus solfataricus

Download Article:
In this work, we have studied the in vitro translational features of a bicistronic mRNA of the extremely thermophilic Archaeon Sulfolobus solfataricus, with the aim of determining the nature of the cis-acting signals controlling the recognition of the translation initiation sites in the Archaea. We found that the most important feature for efficient initiation was the presence of a Shine–Dalgarno (SD)-like ribosome-binding motif, whose disruption entirely abolished the translation of the corresponding cistron. The influence of other features, such as the type of initiation codon, was variable and depended upon the gene and its position in the mRNA. However, the translational block caused by the disruption of the SD sequences could be removed by deleting the 5′ untranslated region altogether, thereby creating a ‘leaderless’ mRNA. This suggests that ‘leaderless’ initiation operates by a default mechanism that does not require a specific mRNA–rRNA interaction and may be common to all three primary domains of life.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Affiliations: Dpt. Biotecnologie Cellulari ed Ematologia, Sez. Genetica Molecolare, Università di Roma ‘La Sapienza’, Policlinico Umberto I, Viale Regina Elena 324, 00161 Rome, Italy.,

Publication date: October 1, 1999

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more