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Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase

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The conditions are described in which DNA replication can occur, in the absence of protein synthesis, in wild-type Escherichiacoli cells. Chromosome replication, which is normally inhibited by addition of chloramphenicol, becomes resistant to this drug after nutritional shiftup, e.g. from minimal medium to Luria broth. This replication activity appears transiently when nutritionally upshifted cells enter stationary phase. The activity strictly requires recA+, but it is independent of recB+ and dnaA+. It can occur in the absence of concomitant transcription. Activation of the replication does not result from induction of the SOS response. As the characteristics of this DNA replication resemble those of the previously characterized stable DNA replication, it is termed nutritional shiftup-activatable stable DNA replication, nSDR. Possible mechanisms of the activation of nSDR in rapidly growing cells at the time of entry to stationary phase are discussed.
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Document Type: Research Article

Affiliations: Departments of Cell Biology and Microbiology and Cancer Center, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA.

Publication date: September 1, 1996

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