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Deletions of Tn916‐like transposons are implicated in

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Summary

Using the tetM‐containing conjugative transposon Tn916 as a mutagenesis tool, we identified two distinct classes of transposon insertions in the meningococcal chromosome. Class I Insertions have an intact copy of Tn916 that appears to have transposed by a novel recombinational mechanism, similar to the transposition of conjugative transposons in Gram‐positive bacteria. Class II insertions were characterized by deletions of Tn916 but preservation of the tetM determinant. In addition, we identified Class II Tn916‐like insertions in the naturally occurring 25.2 MDa tetM‐containing plasmids of both Neisseria meningitidis and Neisseria gonorrhoeae. The truncated Tn916 like insertions appeared to be present in the same site in these two plasmids; however, the deletions of the transposon were different. Plasmid sequence adjacent to the truncated transposon in the 25.2 MDa plasmids was found in a tetracycline‐sensitive N. gonorrhoeae 24.5 MDa conjugative plasmid. These data suggest that the 25.2 MDa plasmids are the result of one or a series of Class II Tn916‐like insertions into 24.5 MDa conjugative plasmids. Class II insertions of Tn916‐like transposons are implicated in the dissemination of tetM resistance in pathogenic Neisseria.
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Document Type: Research Article

Affiliations: 1: Departments of Medicine and Microbiology and Immunology, Emory University School of Medicine, and Laboratories of Microbial Pathogenesis, Research Service, Department of Veterans Affairs Medical Center, 1670 Clairmont Road, Atlanta, Georgia 30033, USA. 2: Departments of Medicine and Microbiology and Immunology, Emory University School of Medicine, and Laboratories of Microbial Pathogenesis, Research Service, Department of Veterans Affairs Medical Center, 1670 Clairmont Road, Atlanta, Georgia 30033, USA.

Publication date: October 1, 1993

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