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Microsatellite markers in western hemlock [Tsuga heterophylla (Raf.) Sarg]

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Abstract

Fifteen microsatellite markers have been isolated from western hemlock [Tsuga heterophylla (Raf.) Sarg] genomic DNA and six of these markers were optimized for use in mountain hemlock [Tsuga mertensiana (Bong) Carr.]. The mean expected heterozygosity (HE) was 0.88 and 0.89 for western hemlock and mountain hemlock, respectively. Allelic diversity was high for both hemlock species, ranging from 7 to 15 alleles per locus for western hemlock and 5–30 alleles per locus for mountain hemlock. The allelic variation identified in this project will allow monitoring of changes in genetic variation as a result of selection pressure in breeding programs.
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Keywords: Tsuga heterophylla; Tsuga mertensiana; cross-species; microsatellite; mountain hemlock; western hemlock;  conifer

Document Type: Research Article

Affiliations: Department of Forest Sciences, 2424 Main Mall, University of British Columbia, Vancouver British Columbia, V6T 1Z4, Canada

Publication date: 01 October 2003

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