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DNA Polymorphism Among Yewei B, V20B, and Oryza minuta J. S. Presl. ex C. B. Presl

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Abstract:

The new cytoplasmic male sterile (CMS) line Yewei A and its maintainer line Yewei B, with better agronomic characteristics, have been developed from a mutant of V20B (a rice maintainer line) through transformation of genomic DNA of wild rice (Oryza minuta J. S. Presl. ex C. B. Presl.). Analysis of molecular markers, DNA sequences, and Southern blot revealed that high DNA polymorphism exists between the mutant and its receptor, indicating that the special DNA fragment from O. minuta may be integrated into the genome of Yewei B. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for creating a new rice germplasm.

(Managing editor: Li-Hui ZHAO)
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Keywords: DNA sequence; genomic DNA introduction; germplasm; molecular markers; wild rice

Document Type: Research Article

Affiliations: 1: China National Hybrid Rice R & D Center, Changsha 410125, China 2: Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100010, China 3: Tianjin Agriculture College, Tianjin 300384, China

Publication date: December 1, 2005

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