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Karyotyping of Brassica napus L. Based on C0t-1 DNA Banding by Fluorescence In Situ Hybridization

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Abstract:

In order to precisely recognize and karyotype Brassica napus L. chromosomes, C0t-1 DNA was extracted from its genomic DNA, labeled withbiotin-11-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization (FISH) signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both C0t-1 DNA FISH banding patterns and chromosome morphology.

(Managing editor: Wei WANG)
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Keywords: Brassica napus; C0t-1 DNA; chromosome banding; fluorescence in situ hybridization (FISH); karyotyping

Document Type: Research Article

Affiliations: 1: Institute of Oil Crops, Chinese Academy of Agricultural Sciences, Wuhan 430062, China 2: College of Life Sciences, Xinyang Normal University, Xinyang 464000, China 3: Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China

Publication date: December 1, 2005

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