Linear phase imaging using differential interference contrast microscopy
We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.
Keywords: Differential interference contrast microscopy; Fourier transform; Hilbert transform; Nomarski differential interference contrast; image processing; phase retrieval; phase shifting; spiral phase transform
Document Type: Short Communication
Affiliations: 1: Physical Optics Laboratory, School of Physics, University of Sydney, NSW, 2006, Australia 2: Canon Information Systems Research Australia, 1 Thomas Holt Drive, North Ryde, NSW, 2113, Australia 3: Department of Applied Physics, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 4: Department of Electrical & Computer Engineering, University of Colorado, Boulder, CO 80309-0425, U.S.A.
Publication date: 01 April 2004