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Linear phase imaging using differential interference contrast microscopy

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We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.
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Keywords: Differential interference contrast microscopy; Fourier transform; Hilbert transform; Nomarski differential interference contrast; image processing; phase retrieval; phase shifting; spiral phase transform

Document Type: Short Communication

Affiliations: 1: Physical Optics Laboratory, School of Physics, University of Sydney, NSW, 2006, Australia 2: Canon Information Systems Research Australia, 1 Thomas Holt Drive, North Ryde, NSW, 2113, Australia 3: Department of Applied Physics, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 4: Department of Electrical & Computer Engineering, University of Colorado, Boulder, CO 80309-0425, U.S.A.

Publication date: April 1, 2004

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