Skip to main content
padlock icon - secure page this page is secure

A Grass Molecular Identification System for Forensic Botany: A Critical Evaluation of the Strengths and Limitations

Buy Article:

$52.00 + tax (Refund Policy)


Plant material is frequently encountered in criminal investigations but often overlooked as potential evidence. We designed a DNA-based molecular identification system for 100 Australian grasses that consisted of a series of polymerase chain reaction assays that enabled the progressive identification of grasses to different taxonomic levels. The identification system was based on DNA sequence variation at four chloroplast and two mitochondrial loci. Seventeen informative indels and 68 single-nucleotide polymorphisms were utilized as molecular markers for subfamily to species-level identification. To identify an unknown sample to subfamily level required a minimum of four markers or nine markers for species identification. The accuracy of the system was confirmed by blind tests. We have demonstrated “proof of concept” of a molecular identification system for trace botanical samples. Our evaluation suggests that the adoption of a system that combines this approach with DNA sequencing could assist the morphological identification of grasses found as forensic evidence.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: chloroplast DNA; forensic botany; forensic science; grasses; indels; mitochondrial DNA; molecular identification system; polymerase chain reaction; single nucleotide polymorphisms; species identification

Document Type: Research Article

Affiliations: 1: National Manager, Forensic and Data Centres, Australian Federal Police, GPO Box 401, Canberra, ACT 2601, Australia. 2: School of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia.

Publication date: November 1, 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more