A Fatal Dichlorvos Poisoning: Concentrations in Biological Specimens
A 54-year-old man was found dead with a bottle containing a brownish fluid near him. A toxicological screening was carried out in blood, urine, and stomach content. Only dichlorvos (2,2 dichlorovinyl O-O dimethylphosphate or DDVP) was found. A simple and rapid method, using DDVP-D6 as an internal standard, was developed for the determination of DDVP by gas chromatography/mass spectrometry (GC/MS). The method was linear from 1 to 10 mg/L. Intraday and interday precisions were all <15%. DDVP concentration in cardiac blood was approximately four times higher than in peripheral blood. A high concentration was found in the heart showing a cardiac tropism of DDVP, kidney and lung concentrations being much lower. No DDVP was found in liver. DDVP stomach content was 38 g. The amount presumed ingested was 82 g, c. 1000 mg/kg of body. The oral LD50 for DDVP ranges between 20 and 1090 mg/kg in animals but is not known for humans.
Document Type: Research Article
Affiliations: Laboratoire de Pharmacologie—Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, AP-HP, et Université Versailles Saint Quentin, 104 Boulevard Raymond. Poincaré, 92380 Garches, France.
Publication date: July 1, 2008