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Dual Examinations for Identification of Urine as Being of Human Origin and for DNA-Typing from Small Stains of Human Urine

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Concurrent methods for identification of urine as being of human origin, and for DNA-typing from small stains of human urine were examined. A urine stain was extracted with phosphate-buffered saline (PBS), and the extract was filtered using a Centricon-100 device. The filtrate was subjected to electrospray ionization liquid chromatography-mass spectrometry (ESI-LC-MS) for identification of human urine and a DNA-typing sample was obtained by dialfiltration of the residue using a DNA purification kit. After the purified residue was treated with an AmpflSTR® Profiler™ PCR amplification kit, the DNA-types were analyzed by capillary electrophoresis using a Genetic Analyzer. It was possible to identify a urine stain as being of human origin, and complete DNA profiles could be successfully obtained from a urine stain which had been created by 50 μL of female urine. Serial analyses of urine stains found at a crime scene provide effective information for forensic investigation. This method is recommended for stain identification and for DNA-typing from a urine stain.
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Keywords: DNA typing; forensic investigation; forensic science; urine stain

Document Type: Research Article

Affiliations: 1: Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, 7-7 Higashikoen, Hakata-ku, Fukuoka 812-8576, Japan. 2: Department of Forensic Medicine, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan. 3: Division of Biological Substance and Life Science, Department of Engineering, Kyushu Kyoritsu University, 1-8 Jiyugaoka, Yahatanishi-ku, Kitakyushu 807-8585, Japan. 4: Institute of Legal Medicine, University Hospital Hamburg-Eppendorf, Butenfeld 34, 22529 Hamburg, Germany.

Publication date: March 1, 2008

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