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Quantifiler™ Observations of Relevance to Forensic Casework

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The Quantifiler™ (QF) kit is regularly used by forensic scientists for DNA quantitation. We performed in-house validation studies which revealed some interesting observations. The QF standard displayed a two-fold difference between two different lot numbers which suggests that every standard should be tested prior to use. The Promega K562 DNA standard works well with the QF kit. c. 41% of samples that inhibited the internal PCR control (IPC) system within the QF kit still produced good Profiler Plus™ reactions. QIAquick® was effective at removing inhibitors. The presence of dyes within casework samples were observed not to inhibit QF amplifications. Template DNA greater than 100 ng/μL appeared to inhibit the IPC. Close to identical concentration results were obtained when alternative analysis settings were used. These validation findings will assist DNA processes involved in forensic casework.
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Keywords: DNA quantification; Quantifiler; forensic DNA analysis; forensic science; multiplex PCR; polymerase chain reaction; real-time PCR

Document Type: Research Article

Affiliations: Victoria Police Forensic Services Centre, Macleod, Vic. 3085, Australia.

Publication date: January 1, 2008

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