Skip to main content
padlock icon - secure page this page is secure

Myotube production in fast myotomal muscle is switched-off at shorter body lengths in male than female Atlantic halibut Hippoglossus hippoglossus (L.) resulting in a lower final fibre number

Buy Article:

$47.00 + tax (Refund Policy)

A sampling method is described to determine accurately the number of fast myotomal muscle fibres (NF) in a large flatfish species, the Atlantic halibut Hippoglossus hippoglossus. An unusual feature of the fast myotomal muscle is the presence of internalized strips of slow muscle fibres. In fish of 1·5–3·5 kg (n = 24), the total cross-sectional area (ATC) of fast muscle was 18% greater in the dorsal than ventral myotomal compartments (P < 0·05), whereas there was no significant difference between left- and right-hand sides of the body. Due the bilateral asymmetry, muscle blocks (5 × 5 × 5 mm) were prepared to systematically sample each myotomal quadrant (dorsal, ventral, left- and right-side) and the diameters of 150 fast fibres measured per block. Smooth non-parametric probability functions were fitted to a minimum of 800 measurements of fibre diameter per quadrant (n = 5). There were no significant differences in the distribution of muscle fibre diameters between myotomal compartments and therefore NF could be estimated from a single quadrant. The number of blocks required to estimate NF with a repeatability of ±2·5% increased from six at 300 g body mass to 17 at 96·5 kg, caused by variation within and between blocks. Gompertz curves were fitted to measurements of fibre number and fork length (LF). The estimated final fibre number was 8·96 × 105 (7·99–9·94 × 105, 95% CI) for males and 1·73 × 106 (1·56–1·90 × 106, 95% CI) for female fish. The estimated LF for cessation of fibre recruitment in the fast muscle of female fish (1775 mm) was almost twice that in males (810 mm), reflecting their greater ultimate body size.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: maximum fibre number; muscle fibre recruitment; muscle fibre types; muscle growth

Document Type: Regular Paper

Affiliations: 1: Gatty Marine Laboratory, School of Biology, University of St Andrews, Fife, Scotland, KY16 8LB, U.K. 2: Faculty of Biosciences and Aquaculture, Bodø University College, NO-8049 Bodø, Norway

Publication date: 01 July 2008

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more