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Open Access Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica

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Abstract Aims: 

To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. Methods and Results: 

In the 5-l stirred-tank bioreactor (STR), with the 0·5 vvm aeration rate, an agitation rate of 200 rev min−1 significantly enhanced the spore yield compared to the same in 300 rev min−1 cultivations. In a 20-l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2·5 vvm operated in a 24-mesh net-draft tube mode, and no pH control cultivation. This spore yield in the 20-l ALR was five- and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5-l STR cultivations respectively. Conclusions: 

Although culture conditions are still to be optimized, by using an ALR with net-draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible. Significance and Impact of the Study: 

The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica.
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Keywords: Bacillus amyloliquefaciens; Botrytis elliptica; airlift bioreactor; antagonism; spore production

Document Type: Research Article

Affiliations: 1:  Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, Republic of China 2:  Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, Republic of China 3:  Department of Horticulture and Biotechnology, Chinese Culture University, Taipei, Taiwan, Republic of China

Publication date: 01 May 2008

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