Skip to main content
padlock icon - secure page this page is secure

Open Access Effect of various environmental factors on the viability of Cryptosporidium parvum oocysts

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract Aims: 

To evaluate individual and combined effects of temperature (4, 18 and 25°C), pH (7 and 10), ammonia (5 and 50 mg l−1) and exposure time (1, 2, 4 and 6 days) on the viability of Cryptosporidium parvum oocysts in water. Methods and Results: 

The viability of oocysts was evaluated using the fluorogenic vital dyes assay (4′,6-diamidino-2-phenylindole and propidium iodide). All the factors analysed (temperature, pH, ammonia and exposure time) and their interaction were statistically significant (P < 0·005). Exposure of oocysts to pH 10 for 6 days at 25°C reduced oocyst viability from ∼80% to 51%. Similarly, the exposure of C. parvum oocysts to 5 mg NH3 l−1 and 50 mg NH3 l−1 for 4 days reduced their viability from between ∼80% to 41·5% and 14·8%, respectively. Conclusions: 

The interaction between pH, temperature and exposure time may have adverse effects on the survival of C. parvum oocysts in water. Low concentrations of ammonia, as commonly found in alga-based wastewater systems, over a long period of time can produce high C. parvum oocyst inactivation rates. Significance and Impact of the Study: 

This study provides relevant data on the inactivation of C. parvum oocysts in alga-based wastewater-treatment systems in the northwest of Spain.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Cryptosporidium parvum; ammonia; pH; temperature; viability; water

Document Type: Research Article

Affiliations: 1:  Department of Ecology, Faculty of Biological and Environmental Sciences, University of León, León, Spain 2:  Scottish Parasite Diagnostic Laboratory, Stobhill Hospital, Glasgow, Scotland, United Kingdom

Publication date: 01 April 2008

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more