Skip to main content
padlock icon - secure page this page is secure

Free Content Co-operative action of interleukin-10 and interferon-γ to regulate dendritic cell functions

Download Article:
 Download
(PDF)
 
Summary

Interleukin-10 (IL-10) and interferon-γ (IFN-γ) double producer is found in a subpopulation of T regulatory type 1 (Tr1) and T helper type 1 (Th1) cells. Consequently, it is of interest how IL-10 and IFN-γ influence the immune system. However, few studies have addressed the co-operative action of these ‘immunosuppressive’ and ‘immunostimulatory’ cytokines. Here, we examine the effect of IL-10 combined with IFN-γ on dendritic cell (DC) functions. Murine bone marrow-derived conventional DCs were stimulated with IL-10 and/or IFN-γ for 24 hr. Tumour necrosis factor-α and IL-12 p40 production by DCs treated with both IL-10 and IFN-γ was significantly lower than that by DCs treated with IL-10 or IFN-γ alone. Major histocompatibility complex class II expression on DCs treated with both cytokines was attenuated compared with that on DCs treated with either cytokine alone. In contrast, levels of inducible nitric oxide synthase and indoleamine 2,3-dioxygenase, which appear to suppress T-cell responses and promote tolerance, in DCs treated with both cytokines were higher than those in DCs treated with IL-10 or IFN-γ alone. Simultaneous treatment with IL-10 and IFN-γ significantly suppressed the ability of DCs to activate CD4+ T cells compared with treatment with either cytokine. Therefore, IL-10 and IFN-γ co-operatively suppress the immunostimulatory functions of DCs.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: activation; cytokines; dendritic cells

Document Type: Research Article

Publication date: July 1, 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more