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Increased division of αβ TCR+ and γδ TCR+ intestinal intraepithelial lymphocytes after oral administration of cholera toxin

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Cholera toxin (CT) or its subunits were given orally to mice and division of intestinal intraepithelial lymphocytes (IEL) in vivo measured by double immunofluorescence using 5-bromo-2′-deoxyuridine (BRdU) and membrane αβ T-cell receptors (TCR) or γδ TCR+ staining in frozen sections. Cholera toxin (10 μg) produced a two- to eightfold-increase in the uptake of BRdU in αβ TCR+ IEL in the duodenum and a two- to fivefold increase in γδ TCR+ IEL in the ileum. Increased uptake of BRdU was also seen after a dose of 100 μg of CT, but this dose was also associated with the loss of αβ TCR+ IEL and γδ TCR+ IEL in the duodenum. CT-A and CT-B subunit produced increased BRdU incorporation by αβ TCR+ in the duodenum and by γδ TCR+ IEL in the ileum. Cholera toxin therefore appears to be mitogenic for IEL, probably due to an indirect mechanism.
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Document Type: Research Article

Affiliations: 1: Department of Paediatric Gastroenterology, St. Bartholomew's Hospital, London, 2: Department of Immunology, Babraham Institute, Babraham, Cambridge UK

Publication date: September 1, 1996

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