N‐Acetylglucosamine modulates function of the skin fibroblasts
Fibroblasts are an important component of the skin determining its properties. N‐Acetylglucosamine (NAG) is the substrate for hyaluronan synthesis, and it also has anti‐inflammatory and anti‐senescent activity in mesothelial cells.
We tested in in vitro‐cultured human skin fibroblasts how supplementation of culture medium with NAG 10 mmol L−1 changes properties of these cells.
Fibroblasts cultured in presence of NAG produced more proteins and that was mainly due to increased synthesis of collagen (+33% vs. control, P < 0.05). Hyaluronan synthesis was increased (+107% vs. control, P < 0.001), but interleukin‐6 synthesis was reduced (−22% vs. control, P < 0.05). Fibroblasts cultured in medium with NAG 10 mmol L−1 demonstrated improved ability to heal the injured layer of cells (+34% vs. control, P < 0.05). Additionally senescence of fibroblasts undergoing replicative ageing in the presence of NAG was less pronounced, as reflected by smaller increase in the population doubling time (−70% vs. control, P < 0.05).
We conclude that NAG induced changes in the skin fibroblasts' properties maybe important for prevention of the age‐dependent changes in its structure and function.
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Document Type: Research Article
Publication date: October 1, 2013