Skip to main content
padlock icon - secure page this page is secure

Identification and quantitation of vitamins K1 and K3 in cosmetic products for facial skin protection

Buy Article:

$52.00 + tax (Refund Policy)

J. Cosmet. Sci., 59, 459–467 (November/December 2008) Synopsis

A simple and rapid analytical method was developed for the determination of vitamins K1 and K3 in facial anti-rash creams. The procedure is based on an ultrasonic extraction of the cosmetic sample with dimethylacetamide, in the presence of an internal standard, followed by HPLC separation. HPLC was performed using a C18 column and spectrophotometric detection at 333 nm. A linear gradient elution was carried out starting with 50% acetonitrile–methanol (75:25 v/v) and water up to 100% acetonitrile–methanol for 5 min. Linearity was established over the concentration range from 0.2 to 1.0 mg mL-1 for vitamin K1 and from 0.02 to 0.1 mg mL-1 for vitamin K3, with LOD values of 100 ng and 20 ng injected, respectively. The accuracy was verified by spiking experiments on model cosmetic samples. The proposed method has been successfully applied for the analysis of commercial samples of creams.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Abstract

Affiliations: 1: Istituto Superiore di Sanita‘, Dipartimento del Farmaco, Viale Regina Elena 299, 00161 Rome 2: Dipartimento di Chimica Fisica ed Inorganica, Universit√° di Bologna, Viale Risorgimento 4, 40136 Bologna, Italy

Publication date: December 1, 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more