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An evaluation of extracts of five traditional medicinal plants from Iran on the inhibition of mushroom tyrosinase activity and scavenging of free radicals

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Synopsis This study aimed to evaluate the free radical scavenging and inhibition properties of five medicinal plants, including Olive., Retz. L., L., L., toward the activity of mushroom tyrosinase usingl-tyrosine andl-3,4-dihydroxyphenylalanine (l-DOPA) as the substrate. The methanol extracts of and showed strong radical scavenging effect in 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay (IC = 15.3 and 82.2 μg mL respectively). These plants also showed inhibitory effects against the activity of mushroom tyrosinase in hydroxylation ofl-tyrosine (85.9% and 82.2% inhibition, respectively). These two plants also inhibited the oxidation ofl-DOPA similar to kojic acid as positive control (IC = 102.8 and 192.6 μg mL respectively). In general and significantly inhibited tyrosinase activity and DPPH radical. Both activities were concentration-dependant but not in linear manner. It is needed to study the cytotoxicity of these plant extracts in pigment cell culture before further evaluation and moving to conditions.


Le but de cette étude est d’évaluer les propriétés antiradicalaires et les propriétés inhibitrices de l’activité de la tyrosinase de champignons de 5 plantes médicinales : Quercus infectoria Olive, Terminalia chebula Retz., Lavendula stoechas L., Mentha longifolia L., Rheum palmatum L, en utilisant comme substrat lal-Tyrosine et la 3,4-dihydroxyphénylalanine (l-Dopa). Les extraits méthyliques de Qercus Infectoria et Terminalia Chebula montrent un fort effet capteur vis-à-vis des radicaux dans le test au diphénylpicrylhydrazyl (DPPH) avec respectivement un IC 50 de 15,3 et 82,2 μg mL−1. Ces plantes montrent également des effets inhibiteurs de l’activité tyrosinase de champignon lors de l’hydroxylation de lal-Tyrosine (respectivement 85,9 et 82,2% d’inhibition). Ces deux plantes inhibent également l’oxydation de lal-Dopa de façon similaire à l’acide kojique utilisé en tant que témoin positif avec respectivement un IC 50 de 102,8 et 192,6 μg mL−1. De façon générale, Quercus infectoria et Terminalia chebula inhibent de façon significative l’activité tyrosinase et le radical DPPH. Ces deux propriétés sont dépendantes de la concentration mais pas de façon linéaire. Il est nécessaire d’étudier la cytotoxicité de ces extraits de plantes sur des cultures de cellules pigmentaires avant d’entreprendre de nouvelles évaluations et d’entamer des essais in vivo.
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Keywords: DPPH; Quercus infectoria; Terminalia chebula; pigmentation; tyrosinase activity

Document Type: Research Article

Affiliations: 1: Department of Pharmaceutics, Research Center of Pharmaceutics, Kerman University of Medical Sciences 2: Kerman University of Medical Sciences 3: Department of Pharmacognosy, Research Center of Pharmaceutics, Kerman University of Medical Sciences, Kerman, Iran

Publication date: October 1, 2009

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