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Determination of 2-aminoethanol in 2,2’,2’’-nitrilotriethanol by reversed-phase HPLC-UV using o-phthalaldehyde as a pre-column derivatizing reagent

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A measurement method for determination of 2-aminoethanol in 2,2’,2’’-nitrilotriethanol by reversed-phase high-performance liquid chromatography (HPLC) with an ultraviolet detector has been developed using o-phthalaldehyde as a pre-column derivatizing reagent. As a result, the calibration curve of 2-aminoethanol standard solutions was linear over the range of 1.0 × 10 – 5 – 5.0 × 10 – 3 mol L–1 with a correlation coefficient of 0.996 and the detection limit, defined as S/N = 3, was 7.5 × 10 – 7 mol L–1. The relative SD at 5.0 × 10 – 5 mol/ L–1 of 2-aminoethanol standard solution was 6.0% (n = 6). This measurement method was applied to the determination of 2-aminoethanol in several commercial 2,2’,2’’-nitrilotriethanol samples and it was found that both the calibration curve method and the standard addition method based on the peak height are available for the determination of 2-aminoethanol. These results suggest that the method reported here will be useful for determination of 2-aminoethanol in 2,2’,2’’-nitrilotriethanol.
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Document Type: Research Article

Publication date: August 1, 2005

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