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Enhancement of human fibroblast growth and other dermatological effects induced by cell extract from heat-shocked blue green alga (Cyanobacteria)

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It has been known that high amounts of ‘heat shock proteins (HSPs)’ are produced under heat and other environmental stresses in cells. Recently, it was reported that HSP could also be accumulated in some dermal cells to protect the cellular proteins from damage caused by ultraviolet radiation. Here, the authors have focused on thermophilic cyanobacteria and the heat shock treatment. Thermophilic cyanobacteria are known to thrive under extreme environmental conditions, and produce distinctively high amounts of HSP when exposed to higher temperatures. We tested cell extract from heat-shocked cyanobacterial cells on a number of skin-related biological activities and found it significantly effective. The cyanobacterial cells, originally collected from a spa as hot as 55 °C, were cultivated in the laboratory at 50 °C and broken by a freeze-fracture method. The extract thus obtained had a superoxide dismutase (SOD)-like activity and inhibited lipid peroxide formation. We found that the extract obtained from the heat-shocked (at 63 °C) cells showed a much stronger SOD-like activity, while the inhibitory effect on lipid peroxide formation remained almost the same. Furthermore, the SOD-like activity could withstand heat up to 70 °C for as long as 1 h. Most remarkably, supplementing this heat-shocked extract strongly promoted the proliferation of human dermal fibroblast culture. Extracts from the cells grown at a regular temperature (50 °C) did not promote the proliferation of the fibroblasts. The heat-shocked extract was fractionated by ultrafiltration for further analysis. A fraction (molecular weight: 10 000–30 000) has shown the strongest activity so far. These results indicate that the heat-shocked cyanobacterial extract with its increased amounts of HSPs is a promising potent ingredient for anti-aging cosmetic materials.
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Document Type: Abstract

Affiliations: 1: Nippon Shikizai Inc., Tokyo, Japan 2: Saitama University, Saitama, Japan

Publication date: August 1, 2004

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