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Collagenous hydrolysates from untraditional sources of proteins. Reaction condition and the yield of enzymatic hydrolysis of short cattle tendons

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Synopsis The effect of reaction conditions on the yield of enzymatic hydrolysis of short cattle tendons was examined using statistical scheme of factorial experiments 2. The duration of enzymatic purification of the starting material and of purified material denaturation processes, the protease concentration in reaction mixture and hydrolysis time were considered to be the main sources of reaction yield variation. An attempt was made to find the conditions leading to maximal yield of collagen hydrolysate.

French
Résumé

On a étudié l'effet des conditions de réaction sur le rendement de l'hydrolyse enzymatique de tendons courts de bétail en utilisant un plan d'expériences statistique de factorielle 24. On a considéré que la durée de la purification enzymatique du produit de départ et des processus de dénaturation du matériau purifié, la concentration en protéase dans le mélange réactionnel et le temps d'hydrolyse étaient les paramètres majeurs de variation du rendement de réaction. On a effectué un essai de résolution des conditions conduisant au rendement maximal en hydrolysat de collagène.
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Keywords: collagen; enzymatic hydrolysis; reaction conditions optimalization; short cattle tendons

Document Type: Research Article

Affiliations: Protein and Leather Technology Institute, Faculty of Technology, Technical University, Nam. TGM 275, Zlin CZ 762 72, Czech Republic

Publication date: August 1, 2001

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