Skip to main content
padlock icon - secure page this page is secure

Prorenin receptor mediates inflammation in renal ischemia

Buy Article:

$52.00 + tax (Refund Policy)

We hypothesized that PRR contributes to renal inflammation in the 2‐kidney, 1‐clip (2K1C) renal ischaemia model. Male Sprague‐Dawley rats were fed normal sodium diet. Blood pressure (BP) was obtained on days 0 and 28 after left renal artery clipping that reduced renal blood flow by 40%. Renal expression of TNF‐α, COX‐2, NF‐κB, IL‐1β, MCP‐1 and collagen type I were assessed in sham and 2K1C rats with or without left renal administration of scramble or PRR shRNA. At baseline, there were no differences in BP. Compared to sham, MAP significantly increased in clipped animals (sham 102 ± 1.9 vs 2K1C 131.8 ± 3.09 mmHg, P < .05) and was not influenced by scramble or PRR shRNA treatment. Compared to sham and contra lateral (non‐clipped) kidney, there was upregulation in mRNA and protein expression of PRR (99% and 45%, P < .01), TNF‐α (72% and 50%, P < .05), COX‐2 (72% and 39%, P < .05), p‐NF‐κB (92%, P < .05), MCP‐1 (87%, P < .05) and immunostaining of collagen type I in the clipped kidney. These increases were not influenced by scramble shRNA. Compared to 2K1C and scramble shRNA, PRR shRNA treatment in the clipped kidney significantly reduced the expression of PRR (62% and 57%, P < .01), TNF‐α (51% and 50%, P < .05), COX‐2 (50% and 56%, P < .05), p‐NF‐κB by 68% (P < .05), MCP‐1 by 73% (P < .05) and collagen type I respectively. Ang II was increased in both kidneys and did not change in response to scramble or PRR shRNA treatments. We conclude that PRR mediates renal inflammation in renal ischaemia independent of blood pressure and Ang II.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: (pro)renin receptor; fibrosis; inflammation

Document Type: Research Article

Publication date: February 1, 2018

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more