Skip to main content
padlock icon - secure page this page is secure

Effects of ovariectomy and 17β-oestradiol replacement on [Ca2+]i in female rat cardiac myocytes

Buy Article:

$52.00 + tax (Refund Policy)


1. The present study investigated the effects of ovariectomy (OVX) and 17β-oestradiol replacement on [Ca2+]i in rat freshly isolated cardiac myocytes.

2. Myocytes were isolated from the hearts of sham, OVX and OVX + 17β-oestradiol-replaced female rats by enzymatic digestion with collagenase. Changes in [Ca2+]i in response to varied extracellular [Ca2+] were measured using the Ca2+-sensitive dye fura-2, with the contractile responses of each cell measured as cell shortening.

3. Increasing extracellular [Ca2+] resulted in increased [Ca2+]i in all three groups. Peak [Ca2+]i and the amplitude of the Ca2+ transient were significantly greater (P < 0.01) in cells from OVX animals compared with cells from both sham and 17β-oestradiol-replaced OVX animals.

4. The time-course of decay of the Ca2+ transient was significantly faster (P < 0.02) in OVX cells compared with both sham and 17β-oestradiol-replaced cells. In addition, time to 50% relaxation was significantly faster (P < 0.04) and extent of shortening significantly greater (P < 0.01) in OVX cells than in either sham or 17β-oestradiol cells.

5. These data demonstrate clear differences in peak [Ca2+]i and the amplitude of the Ca2+ transient between OVX female rat cardiac myocytes compared with intact and 17β-oestradiol-replaced OVX female rat cardiac myocytes. This suggests that oestrogen may play a long-term role in limiting Ca2+ entry into the cardiac myocyte.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: calcium; cardiac myocytes; gender; oestrogen; ovariectomy

Document Type: Research Article

Affiliations: Department of Physiology, Monash University, Melbourne, Victoria, Australia

Publication date: July 1, 2003

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more