Skip to main content
padlock icon - secure page this page is secure

Inhibition of nuclear translocation of transcription factor nuclear factor-κB induces FAS- as well as tumour necrosis factor-α-mediated apoptosis through downregulation of a conserved family of inhibitor of apoptosis 1

Buy Article:

$52.00 + tax (Refund Policy)

Summary

1. In the present study, we examined whether the nuclear transcription factor (NF)-κB activity plays a role in the determination of sensitivity to tumour necrosis factor (TNF)-α or agonistic Fas antibody (Ab) in human vascular smooth muscle cells (hVSMC).

2. To inhibit agonist-induced NF-κB activation in hVSMC, a cell-permeable peptide (SN50), which carried the nuclear localization sequence of the NF-κB p50 subunit, was used. Nuclear factor-κB activity was examined by both immunoblot analysis of nuclear extracts and by ELISA. The hVSMC were treated with TNF-α or agonistic Fas Ab (CH11) and then apoptosis was determined by cell death ELISA for DNA fragmentation. To investigate the mechanisms for protection against apoptosis in hVSMC, we analysed the expression of a conserved family of inhibitor of apoptosis 1 (c-IAP1) protein using immunoblot analysis.

3. Although both CH11 and TNF-α alone failed to induce hVSMC death in the presence of SN50, they markedly increased the apoptotic hVSMC estimated by cell death ELISA. In addition, these effects could be blocked with the pan-caspase inhibitor z-VAD.fmk. Western blotting analysis indicated that TNF-α alone increased c-IAP1 protein levels, whereas CH11 alone had no effect. Inhibition of NF-κB activation by SN50 suppressed c-IAP1 protein expression and enhanced apoptosis induced by either TNF-α or CH11.

4. These findings suggest that c-IAP1 is an important intracellular modulator of Fas as well as TNF-α death signalling pathways in hVSMC. The expression of c-IAP1 is regulated by a NF-κB-mediated phenomenon.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: apoptosis; atherosclerosis; cell culture; gene expression; isolation; smooth muscle

Document Type: Research Article

Affiliations: Department of Cardiovascular Medicine, Wakayama Medical University, Wakayama, Japan

Publication date: March 1, 2003

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more