Optimisation of combi‐lipases from Aspergillus niger for the synergistic and efficient hydrolysis of soybean oil
The enzymatic properties of four lipases (A, B, C and D) from different strains of Aspergillus niger, were investigated, and a 3‐factor mixture design and triangular surface analysis were performed to screen the optimal combi‐lipase by observing synergistic effects. Lipases B and D differed in optimal pH, temperature and substrate specificity. A combi‐lipase with 31.2% lipase B and 68.8% lipase D (w/w, equal to units of 30.36% and 69.64%) exhibited optimal hydrolytic activity on soybean oil, which exceeded the sum of the combined activities of individual lipases (P < 0.05). Free fatty acid from the hydrolyzed soybean oil indicated that the synergistic effect of the combi‐lipase resulted from the different fatty acid specificities of the two lipases. Overall, combi‐lipase afforded an effective route for the application of lipase enzymes to animal feeds.
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