Skip to main content
padlock icon - secure page this page is secure

Polymorphism of inhibin βB gene and its relationship with litter size in sheep

Buy Article:

$52.00 + tax (Refund Policy)


The inhibin βB (INHBB) gene was studied as a candidate gene for the prolificacy of Small Tail Han and Hu sheep. According to the sequence of exon 1 and 2 of bovine INHBB gene, six pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 and 2 of INHBB gene in both high (Small Tail Han and Hu sheep) and low prolificacy breeds (Dorset, Texel and German Mutton Merino sheep) by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Three pairs of primers (primers 1-1, 1-2 and 1-3) were used to amplify the exon 1, and others (primers 2-1, 2-2 and 2-3) to the exon 2. Only the products amplified by primer 2-3 displayed polymorphism. For primer 2-3, three genotypes (AA, AB and BB) were detected in Hu sheep and only AA genotype in other breeds. In Hu sheep, frequency of AA, AB and BB genotypes was 0.636, 0.046 and 0.318, respectively. Sequencing revealed 276A > G mutation (based on the amplification region of primer 2-3) which did not cause any amino acid change because it lay in the 3′ untranslated region. The ewes with genotype BB had 0.58 (P < 0.01) lambs more than those with AA in Hu sheep.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: PCR-SSCP; inhibin βB gene; prolificacy; sheep

Document Type: Research Article

Affiliations: 1: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 2: College of Animal Science and Technology, Hebei Agricultural University, Baoding 3: College of Life Science, Liaoning Normal University, Dalian, China 4: Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing

Publication date: February 1, 2011

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more