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Calf production from vitrified bovine sexed embryos following in-straw dilution

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ABSTRACT

The objective of this study was to develop an in-straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified-warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in-straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in-straw dilution (Non-evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non-evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in-straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in-straw method and that the vitrified and warmed sexed embryos can develop to term.
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Keywords: bovine; direct transfer; in-straw dilution; sexed embryo; vitrification

Document Type: Research Article

Affiliations: 1: Fukushima Agricultural Technology Centre, Fukushima, Fukushima, 2: Niigata Agricultural Reserch Institute, Sanjo, Niigata, 3: Tochigi Prefectural Dairy Farming Experiment Station, Shiobara, Tochigi, 4: Ishikawa Livestock Reseach Center, Houdatsushimizu, Ishikawa, 5: Shimane Prefectural Livestock Technical Center, Izumo, Shimane, 6: Okayama Prefectural Center of Animal Husbandry and Research, Misaki, Okayama, 7: Yamaguchi Prefectural Livestock Research Institute, Mine, Yamaguchi, 8: Hukuoka Agricultural Research Center, Chikushino, Fukuoka, 9: Miyazaki Livestock Research Institute, Takaharu, Miyazaki and 10: National Livestock Breeding Center, Nishigo, Fukushima, Japan

Publication date: August 1, 2010

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