Skip to main content
padlock icon - secure page this page is secure

Molecular cloning, chromosomal localization and expression pattern of porcine ADP-ribosylation factor(Arf) gene family

Buy Article:

$52.00 + tax (Refund Policy)


Adenosine diphosphate-ribosylation factors (Arfs) are a family of guanosine triphosphate-binding proteins involved in fundamental biological processes including secretion, endocytosis, phagocytosis, cytokinesis, cell adhesion and tumor cell invasion. We report here the molecular cloning, chromosome localization and expression analysis of porcine Arf1–6, of which Arf1–3 (Class I) have >93% similarity to each other and encode nearly similar proteins with 181 amino acids in length. Arf4 and Arf5 (Class II) are 78–81% homologous to Class I Arfs and both encode a protein of 180 amino acids, Arf6 (Class III) shows 64–68% homology to the other Arfs and encodes 175 amino acids. With radiation hybrid mapping, porcine Arf1–6 are assigned to chromosomes 14q21-q22, 12p14, 5p12-q11, 13q21.1, 18q24, 1q21-q27, respectively. Moreover, real-time quantitative RT-PCR assays show that porcine Arf1-6 are ubiquitous in all tissues examined, with the highest levels in the kidney and stomach and the lowest in muscle and the heart. This is the first report of molecular characterization of the Arf gene family in pigs.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Arfs; chromosomal localization; cloning; expression pattern

Document Type: Research Article

Publication date: August 1, 2010

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more