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Mechanisms involved in the cytosolic calcium ion elevation induced by activating factors in chicken and rat phagocytes

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The aim of the present study was to determine the mechanism of cytosolic calcium ion concentration [Ca2+]i elevation in chicken and rat phagocytes stimulated with phorbol myristate acetate (PMA), leukotriene B4 (LTB4), formyl-methionyl-leucyl-phenylananine (fMLP) and Saccaromyces cerevisiae culture supernatant (SCS). Pretreatment with EGTA completely suppressed the PMA-induced [Ca2+]i elevation in rat and chicken phagocytes, suggesting that all the [Ca2+]i elevation induced in the PMA-stimulated rat and chicken phagocytes was attributable to the influx of extracellular Ca2+. On the other hand, the elevation of LTB4-, FMLP- and SCS-induced [Ca2+]i was only partially suppressed by ethyleneglycol-bis (β-aminoethyl)-N,N,N′,N′-tetraacetic acid ethylene (EGTA) pretreatment of phagocytes. The results indicated that two pathways of [Ca2+]i elevation, recruitment from the intracellular Ca2+ store and influx of extracellular Ca2+, are involved in the [Ca2+]i elevation of LTB4-, fMLP- and SCS-stimulated phagocytes. In fMLP-stimulated rat neutrophils, [Ca2+]i elevation showed a two-phase pattern in which the time lag between the first and second phase was approximately 1 min. The EGTA treatment of the fMLP-stimulated cells induced a reduction of the first phase level and a disappearance of the second phase. The reason for the special influence of EGTA observed in fMLP-stimulated cells is unknown, but the disappearance of the second phase of the [Ca2+]i may be elicited by the EGTA-induced decrease of the first phase [Ca2+]i elevation that depends on IP3 and diacylglycerol induced by fMLP.
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Keywords: chicken phagocyte; cytosolic calcium ion; formyl-methionyl-leucyl-phenylananine; leukotriene B4; phorbol myristate acetate

Document Type: Research Article

Affiliations: 1: Chemical Engineering College, Inner Mongolia University of Technology, Huhehaote, China; and 2: Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan

Publication date: February 1, 2007

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