Skip to main content
padlock icon - secure page this page is secure

Temporal expression of growth factor genes of primary porcine satellite cells during myogenesis

Buy Article:

$52.00 + tax (Refund Policy)

ABSTRACT

The present experiment was undertaken to study the transcription of members of the insulin-like growth factor (IGF) system and myostatin in porcine satellite cells through myogenesis and to study the effects of 1 µmol/L insulin on stimulating fusion. Cultured cells were harvested at 50 and 80% confluence and at days 1, 2, 3 and 4 following induction of fusion and differentiation. Increased levels of myogenin transcription and creatine kinase from 50% confluence until day 2 indicated that fusion and differentiation were induced. The mRNA abundance of IGF-I (P < 0.001), IGF-II (P < 0.001), IGF binding protein (BP) 5 (P < 0.01), type I IGF receptor (P < 0.001) and myostatin (P < 0.01) increased from proliferation to differentiation. Insulin stimulated differentiation as indicated by elevated creatine kinase activity (P < 0.001) but did not affect myogenin mRNA abundance (P = 0.98). Insulin downregulated transcription of IGF-I (P < 0.01) and during the first 2 days after switching to differentiation medium insulin downregulated IGF receptor transcription (P < 0.001), whereas insulin did not affect transcription of IGF-II (P = 0.06), IGFBP5 (P = 0.76) or myostatin (P = 0.50). In conclusion, transcriptions of IGF-I, IGF-II, type I IGF receptor, IGFBP5 and myostatin were changed during myogenesis, suggesting autocrine/paracrine roles in regulating the transition from proliferation to differentiation of porcine satellite cells.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: cell culture; gene expression; insulin-like growth factor; pigs; satellite cells

Document Type: Research Article

Publication date: June 1, 2006

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more