Skip to main content
padlock icon - secure page this page is secure

Mapping of expressed sequence tag markers with a cDNA-amplified fragment length polymorphism method in Japanese quail (Coturnix japonica)

Buy Article:

$52.00 + tax (Refund Policy)

ABSTRACT

In our previous study, a Kobe-NIBS Japanese quail (KNQ) linkage map was constructed mainly using amplified fragment length polymorphism (AFLP) markers. In order to compare chicken and quail chromosomes, we developed expressed sequence tag (EST) markers derived from cDNA-AFLP fragments and localized these markers on the linkage map. Using a total of 128 AFLP primer combinations, 24 polymorphic bands were obtained between a neurofilament-deficient mutant quail line male and a muscular disorder quail line female, which were the parents of the KNQ resource family. Nine of the 24 markers were mapped by linkage analysis. These markers were mapped to seven linkage groups, namely 1, 2, 3, 6, 8, 15 and 42. A subsequent homology search using chicken genome sequences strongly suggests that these linkage groups correspond with chicken chromosomes 1, 2, 3, 5, 15, 23 and 26.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Japanese quail; cDNA-amplified fragment length polymorphism; linkage map

Document Type: Research Article

Affiliations: 1: Faculty of Agriculture and 2: Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe-shi and 3: Nippon Institute for Biological Science, Kobuchisawa-machi, Yamanashi-ken, Japan

Publication date: February 1, 2006

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more