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Gene expression of resistin and TNF-α in adipose tissue of Japanese Black steers and Holstein steers

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ABSTRACT

The aim of the present study was to compare the expression of adipose tissue mRNA related to glucose metabolism between Japanese Black steers (n = 5) and Holstein steers (n = 5). We examined the expression of the resistin, tumor necrosis factor-α (TNF-α), glucose transporter 1 (GLUT1) and growth hormone receptor (GHR) genes using real-time polymerase chain reaction of cDNA in adipose tissue. The cDNA sequence identified by 5′/3′-rapid amplification of cDNA and the deduced amino acid sequence were highly conserved in human, porcine and murine resistin. Expression of resistin mRNA was significantly greater in Holstein steers than in Japanese Black steers. In contrast, expression of TNF-α mRNA was slightly greater in Japanese Black steers. Expression of GHR mRNA was significantly greater in Japanese Black steers compared with the Holstein steers, although there was no significant difference in the expression of GLUT1 mRNA. However, the plasma non-esterified fatty acid (NEFA), glucose, insulin and growth hormone concentrations did not differ between Japanese Black and Holstein steers. The present results show that there is a difference in the expression level of mRNA related to glucose metabolism between Japanese Black steers and Holstein steers.
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Keywords: Holstein steers; Japanese Black steers; adipose tissue; resistin; tumor necrosis factor-α

Document Type: Research Article

Affiliations: 1: National Institute of Livestock and Grassland Science, Tsukuba-shi, 2: School of Veterinary Medicine and Animal Science, Kitasato University, Towada-shi and 3: Graduate School of Agricultural Science, Tohoku University, Sendai-shi, Japan

Publication date: December 1, 2005

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