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Effects of supplement feeding order on lactation, diurnal variation of ruminal ammonia and urea in the blood and milk of dairy cows

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The present study examined the effects of feeding order of grain and protein supplements on ruminal nitrogen (N) loss through the diurnal variation of ruminal ammonia N and urea N in the blood and milk of dairy cows. Three ruminally fistulated cows were used in a 3 × 3 Latin square arrangement of treatments; that is, grain supplement fed at the first feed and then the protein supplement at the second (GS-1st), protein supplement fed first and then the grain supplement (PS-1st), and the grain and protein supplements mixed and divided into equal portions fed at each supplement feeding time (GP-mix). No differences in the lactation parameters were observed among the treatments. In GS-1st treatment, the increase in the concentration of ruminal ammonia N was inhibited after feeding, and the concentration was lower (P < 0.05) than with the other treatments 1–2 h after basal feeding. The concentrations of urea N in the blood and milk with the GS-1st treatment were the lowest of all treatments throughout the sampling times, and were lower (P < 0.05) than with the GP-mix treatment except for blood levels 1 h after basal feeding. The results suggest that grain feeding before protein feeding can reduce ruminal N loss.
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Keywords: blood urea; lactation; milk urea; ruminal ammonia; supplement

Document Type: Research Article

Affiliations: 1: Awaji Agricultural Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Mihara-cho, Hyogo-ken, 2: Graduate School of Veterinary Sciences, Gifu University, Gifu-shi and 3: Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University, Morioka-shi, Japan

Publication date: February 1, 2005

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