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Immunohistolocalization and Gene Expression of the Secretory Carbonic Anhydrase Isozymes (CA‐VI) in Canine Oral Mucosa, Salivary Glands and Oesophagus

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The immunohistolocalization of secretory carbonic anhydrase isoenzymes (CA‐VI) in canine salivary glands, parotid, submandibular, sublingual and zygomatic glands, oral and oesophageal mucosa was studied using a specific antiserum against a canine CA‐VI. In addition, the gene expression of CA‐VI from the same tissue was studied using a real‐time reverse‐transcriptase polymerase chain reaction. In all salivary glands and oesophageal gland, immunostaining intensely localized CA‐VI antiserum throughout the cytoplasm of serous acinar cells, including serous demilune and ductal epithelial cells. In contrast, no immunoreaction localized CA‐VI in the mucous acinar cells of the gland. CA‐VI gene transcripts were also detected in the same areas. The physiological significance of secretory CA‐VI in the oral and oesophageal cavity is thought to play a highly specialized role in the maintenance of bicarbonate level in saliva and to protect mucosa from acid injury. It is shown that the major sites of the CA‐VI secretion in dogs were in serous (demilune) secretory cells in all four major salivary glands and oesophageal glands in particular.
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Document Type: Research Article

Affiliations: 1: Addresses of authors: Laboratories of Anatomy I 2: Physiology I 3: Molecular Biology, Azabu University School of Veterinary Medicine, Fuchinobe 1-17-71, Sagamihara, Kanagawa, 229-8501, Japan

Publication date: February 1, 2007

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