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Alpha-latrotoxin Triggers Extracellular Ca2+-dependent Exocytosis and Sensitizes Fusion Machinery in Endocrine Cells

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α-from the venom of black widow spider induces and augments neurotransmitter and hormone release by way of extracellular Ca2+ influx and cellular signal transduction pathways. By using whole cell current and capacitance recording, the photolysis of caged Ca2+, and Ca2+ microfluorometry and amperometry, we investigated the stimulating effect and mechanism of α-latrotoxin on exocytosis in rat pancreatic  cells, LT2 cells and latrophilin plasmid-transfected lNS-1 cells. Our data indicated that: (1) α-latrotoxin increased cytosolic Ca2+ concentration through the formation of cation-permitting pores and subsequent Ca2+ influx with the presence of extracellular Ca2+ (2) α-latrotoxin stimulated exocytosis in normal bath solution and its stimulating effect on secretion was eradicated in Ca2+-free bath solution; and (3) α_ latrotoxin sensitized the molecular machinery of fusion through activation of protein kinase C and increased the response of cells to Ca2+ photolysed by a flash of ultraviolet light. In α latrotoxin induced exocytosis by way of Ca2+ influx and accelerated vesicle fusion by the sensitization of fusion machinery.

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Keywords: Ca2+-sensitivity of fusion; amperometry; calcium; capacitance measurement; exocytosis; protein kinase C (PKC); α-latrotoxin

Document Type: Research Article

Affiliations: School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Publication date: January 1, 2006

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