Skip to main content
padlock icon - secure page this page is secure

Characterization of glycolytic metabolism and ion transport of

Buy Article:

$52.00 + tax (Refund Policy)

Abstract

The main energetic pathways, fermentation and respiration, and the general ion transport properties of Candida albicans were studied. Compared to Saccharomyces cerevisiae, we found that in C. albicans: (a) the cell mass yield when grown in YPD was significantly larger; (b) it required longer times to be starved of endogenous substrates; (c) ethanol production was lower but significant; (d) respiration was also lower; (e) it showed a small activity of an alternative oxidase; (f) fermentation and oxidative phosphorylation seemed to compete for both ADP and NADH; and (g) NADH levels were lower. Regarding ion transport and compared to S. cerevisiae: (a) the general mechanism was similar, with a plasma membrane H+‐ATPase that generates both a plasma membrane ΔpH and a ΔΨ, the latter being responsible for driving K+ inside; (b) its acidification capacity is slightly smaller and less sensitive to activation by high pH; and (c) the presence of K+ results in a large activation of both respiration and fermentation, most probably due to the energy required in the process. ADP produced by H+‐ATPase stimulation by high pH or the addition of K+ at low pH results in the increase of both respiration and fermentation. Copyright © 2012 John Wiley & Sons, Ltd.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: September 1, 2012

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more