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Publisher: Wiley-Blackwell

Volume 34, Number 3, 1 May 2018

Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally‐derived or not
pp. 559-569(11)
Authors: Zhou, Yizhou; Shaw, David; Lam, Cynthia; Tsukuda, Joni; Yim, Mandy; Tang, Danming; Louie, Salina; Laird, Michael W.; Snedecor, Brad; Misaghi, Shahram

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Effective and efficient characterization of Chinese hamster ovary production cell lines using automated intracellular staining and statistical modeling
pp. 570-583(14)
Authors: Krebs, Lara E.; Bowden, Daniel M.; Bray, Christopher M.; Shaw, Margaret M.; Frye, Christopher C.

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Development and characterization of an automated imaging workflow to generate clonally‐derived cell lines for therapeutic proteins
pp. 584-592(9)
Authors: Shaw, David; Yim, Mandy; Tsukuda, Joni; Joly, John C.; Lin, Andy; Snedecor, Brad; Laird, Michael W.; Lang, Steven E.

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Measuring the aggregation of CHO cells prior to single cell cloning allows a more accurate determination of the probability of clonality
pp. 593-601(9)
Authors: Klottrup, Kerensa J.; Miro‐Quesada, Guillermo; Flack, Layla; Pereda, Ivan; Hawley‐Nelson, Pamela

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Open Access Analytical subcloning of a clonal cell line demonstrates cellular heterogeneity that does not impact process consistency or robustness
pp. 602-612(11)
Authors: Scarcelli, John J.; Hone, Megan; Beal, Kathryn; Ortega, Alejaida; Figueroa, Bruno; Starkey, Jason A.; Anderson, Karin

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Open Access Characterization of phenotypic and genotypic diversity in subclones derived from a clonal cell line
pp. 613-623(11)
Authors: Tharmalingam, Tharmala; Barkhordarian, Hedieh; Tejeda, Nicole; Daris, Kristi; Yaghmour, Sam; Yam, Pheng; Lu, Fang; Goudar, Chetan; Munro, Trent; Stevens, Jennitte

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Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality
pp. 624-634(11)
Authors: Ko, Peggy; Misaghi, Shahram; Hu, Zhilan; Zhan, Dejin; Tsukuda, Joni; Yim, Mandy; Sanford, Mark; Shaw, David; Shiratori, Masaru; Snedecor, Brad; Laird, Michael; Shen, Amy

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Antibody expression stability in CHO clonally derived cell lines and their subclones: Role of methylation in phenotypic and epigenetic heterogeneity
pp. 635-649(15)
Authors: Patel, Neha A.; Anderson, Colin R.; Terkildsen, Sarah E.; Davis, Ray C.; Pack, Laura D.; Bhargava, Swapnil; Clarke, Howard R.G.

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Open Access Development of a novel affinity chromatography resin for platform purification of bispecific antibodies with modified protein a binding avidity
pp. 650-658(9)
Authors: Tustian, Andrew D.; Laurin, Linus; Ihre, Henrik; Tran, Travis; Stairs, Robert; Bak, Hanne

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Monitoring mAb cultivations with in‐situ raman spectroscopy: The influence of spectral selectivity on calibration models and industrial use as reliable PAT tool
pp. 659-670(12)
Authors: Santos, Rafael M.; Kessler, Jean‐Michel; Salou, Patrick; Menezes, Jose C.; Peinado, Antonio

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Analysis of Recombinant Human Serum Albumin Extraction and Degradation in Transgenic Rice Extracts
pp. 681-691(11)
Authors: Sheshukova, Kseniya A.; Wilken, Lisa R.

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Rapid fabrication of detachable three‐dimensional tissues by layering of cell sheets with heating centrifuge
pp. 692-701(10)
Authors: Haraguchi, Yuji; Kagawa, Yuki; Hasegawa, Akiyuki; Kubo, Hirotsugu; Shimizu, Tatsuya

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Effects of ultrasonication variables on the activity and properties of alpha amylase preparation
pp. 702-710(9)
Authors: Tran, Thi Thu Tra; Nguyen, Khanh Tien; Le, Van Viet Man

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Surfactant selection for a liquid foam‐bed photobioreactor
pp. 711-720(10)
Authors: Janoska, Agnes; Vázquez, María; Janssen, Marcel; Wijffels, René H.; Cuaresma, María; Vílchez, Carlos

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Development of generic raman models for a GS‐KOTM CHO platform process
pp. 730-737(8)
Authors: Webster, Thaddaeus A.; Hadley, Brian C.; Hilliard, William; Jaques, Colin; Mason, Carrie

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Investigation of the free heavy chain homodimers of a monoclonal antibody
pp. 738-745(8)
Authors: Chung, Hyo (Helen); Buck, Lynette; Daris, Kristi; Welborn, Brent; Luo, Quanzhou; Wypych, Jette

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Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
pp. 746-755(10)
Authors: Morales, Diego; Piris, Adriana J.; Ruiz‐Rodriguez, Alejandro; Prodanov, Marin; Soler‐Rivas, Cristina

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Probing lactate metabolism variations in large‐scale bioreactors
pp. 756-766(11)
Authors: Xu, Sen; Jiang, Rubin; Mueller, Roland; Hoesli, Nadja; Kretz, Thomas; Bowers, John; Chen, Hao

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Physicochemical and catalytic properties of acylase I from aspergillus melleus immobilized on amino‐ and carbonyl‐grafted stöber silica
pp. 767-777(11)
Authors: Kołodziejczak‐Radzimska, Agnieszka; Zdarta, Jakub; Jesionowski, Teofil

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Cryopreservation of chicken blastodermal cells and their quality assessment by flow cytometry and transmission electron microscopy
pp. 778-783(6)
Authors: Svoradová, Andrea; Kuželová, Lenka; Vašíček, Jaromír; Olexíková, Lucia; Chrenek, Peter

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Metabolic responses and pathway changes of mammalian cells under different culture conditions with media supplementations
pp. 793-805(13)
Authors: Park, Seo‐Young; Reimonn, Thomas M.; Agarabi, Cyrus D.; Brorson, Kurt A.; Yoon, Seongkyu

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