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Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white

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A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico‐chemically characterized by ss‐NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g−1 while the dissociation constant was 0.074 ± 0.012 mg mL−1. The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP‐HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:387–396, 2018
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Keywords: chitosan; chromatographic matrix; egg white lysozyme; multimodal ion exchange chromatography; sulfanilic acid

Document Type: Research Article

Publication date: March 1, 2018

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