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Monitoring a Complex Medium Fermentation with Sample‐Sample Two‐Dimensional FT‐NIR Correlation Spectroscopy

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The possibility of monitoring state transitions during the time course of a fermentation through the analysis of the on‐line collected near‐infrared (NIR) spectra with sample‐sample two‐dimensional correlation spectroscopy (SS‐2DCoS) was investigated. SS‐2DCoS has proved to be useful for extracting process information directly from the spectra collected on‐line. The complexity of the system studied prevented the extraction of concentration profiles, but nevertheless, the application of SS‐2DCoS enables the identification of fermentation state transitions due to metabolic and morphological changes of the microorganism.
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Document Type: Research Article

Affiliations: Centre for Biological and Chemical Engineering, IST, Technical University of Lisbon, Av. Rovisco Pais, P-1049–001 Lisbon, Portugal

Publication date: January 1, 2006

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