Skip to main content
padlock icon - secure page this page is secure

Use of Fluorescent Probes for Determination of Yeast Cell Viability by Gravitational Field‐Flow Fractionation

Buy Article:

$59.00 + tax (Refund Policy)

The quality of wine greatly depends on the features of the yeast used in its production, and yeast cell viability is one of the most important quality control issues to consider in this regard. In the first steps of winemaking, the use of a low‐cost and simple methodology for monitoring the cell viability of yeast inoculates is of paramount importance. Gravitational field‐flow fractionation is a useful technique for the determination of cell viability because it provides gentle experimental conditions, although the proper use of fluorophore probes as biomass indicators is required. In this paper the use of different fluorescent probes such as carboxyfluorescein diacetate (cFDA), calcein‐AM, and SYTO‐13 were considered as viability biomarkers. Calceina‐AM allowed the establishment of a direct GrFFF method to determine cell viability, with a limit of detection of 5.0 × 104 viable cell/mL. SYTO‐13 could be used as biomass indicator with a limit of detection of 3.5 × 104 total cells/mL. The suitability of the procedure was tested with three commercial yeast samples, and the results were compared with those obtained using standard techniques.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: Departament de Química Analítica, Universitat de Barcelona, Martí i Franquès, 1–11, 08028 Barcelona, Spain 2: Departament de R+D+I, Uban Research, Parc Científic de Barcelona, Baldiri Reixac, 4–6, Torre D. Barcelona, Spain

Publication date: January 1, 2006

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more