Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray diffraction analysis of YisP protein from

Buy Article:

$52.00 + tax (Refund Policy)

YisP is an enzyme involved in the pathway of biofilm formation in bacteria and is predicted to possess squalene synthase activity. A BlastP search using the YisP protein sequence from Bacillus subtilis subsp. subtilis strain 168 shows that it shares 23% identity with the dehydrosqualene synthase from Staphylococcus aureus. The YisP from B. subtilis 168 was expressed in Escherichia coli and the recombinant protein was purified and crystallized. The crystals, which belong to the orthorhombic space group P212121, with unit‐cell parameters a = 43.966, b = 77.576, c = 91.378 Å, were obtained by the sitting‐drop vapour‐diffusion method and diffracted to 1.92 Å resolution. Structure determination using MAD and MIR methods is in progress.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: January 1, 2013

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more