Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray crystallographic analysis of UDP‐glucuronic acid:flavonol‐3‐

Buy Article:

$52.00 + tax (Refund Policy)

Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP‐glucuronic acid:flavonol‐3‐O‐glucuronosyltransferase that catalyses the 3‐O‐specific glucuronosylation of flavonols using UDP‐glucuronic acid as a sugar donor to produce flavonol 3‐O‐glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting‐drop vapour‐diffusion method. A full set of X‐ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron‐radiation source. The crystal was hexagonal, belonging to space group P6122, with unit‐cell parameters a = b = 102.70, c = 535.92 Å. The initial phases were determined by the molecular‐replacement method.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: January 1, 2013

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more