Skip to main content
padlock icon - secure page this page is secure

Crystallization and preliminary X‐ray diffraction studies of

Buy Article:

$52.00 + tax (Refund Policy)

Regulator of G‐protein signalling (RGS) proteins negatively regulate heterotrimeric G‐protein signalling through their conserved RGS domains. RGS domains act as GTPase‐activating proteins, accelerating the GTP hydrolysis rate of the activated form of Gα‐subunits. Although omnipresent in eukaryotes, RGS proteins have not been adequately analysed in non‐mammalian organisms. The Drosophila melanogaster Gαo‐subunit and the RGS domain of its interacting partner CG5036 have been overproduced and purified; the crystallization of the complex of the two proteins using PEG 4000 as a crystallizing agent and preliminary X‐ray crystallographic analysis are reported. Diffraction data were collected to 2.0 Å resolution using a synchrotron‐radiation source.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Document Type: Research Article

Publication date: January 1, 2013

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more