Preliminary crystallographic analysis of a polyadenylate synthase from
Megavirus chilensis, a close relative of the Mimivirus giant virus, is also the most complex virus sequenced to date, with a 1.26 Mb double‐stranded DNA genome encoding 1120 genes. The two viruses share common regulatory elements such as a peculiar palindrome governing the termination/polyadenylation of viral transcripts. They also share a predicted polyadenylate synthase that presents a higher than average percentage of residue conservation. The Megavirus enzyme Mg561 was overexpressed in Escherichia coli, purified and crystallized. A 2.24 Å resolution MAD data set was recorded from a single crystal on the ID29 beamline at the ESRF.
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Document Type: Research Article
Affiliations: Information Génomique et Structurale, IGS UMR7256, CNRS, Aix-Marseille Université, IMM, FR3479, 163 Avenue de Luminy – case 934, 13288 Marseille CEDEX 09, France
Publication date: January 1, 2013