Crystallization and preliminary diffraction studies of SFC‐1, a carbapenemase conferring antibiotic resistance
SFC‐1, a class A carbapenemase that confers antibiotic resistance, hydrolyzes the β‐lactam rings of β‐lactam antibiotics (carbapenems, cephalosporins, penicillins and aztreonam). SFC‐1 presents an enormous challenge to infection control, particularly in the eradication of Gram‐negative pathogens. As SFC‐1 exhibits a remarkably broad substrate range, including β‐lactams of all classes, the enzyme is a potential target for the development of antimicrobial agents against pathogens producing carbapenemases. In this study, SFC‐1 was cloned, overexpressed, purified and crystallized. The SFC‐1 crystal diffracted to 1.6 Å resolution and belonged to the orthorhombic space group P212121, with unit‐cell parameters a = 65.8, b = 68.3, c = 88.8 Å. Two molecules are present in the asymmetric unit, with a corresponding V M of 1.99 Å3 Da−1 and a solvent content of 38.1%.
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Document Type: Research Article
Publication date: September 1, 2012